| Title |
ESTs from late stage developing seeds of Medicago truncatula |
| Library name |
GLSD |
| Library type |
EST, 5'-3' unidirectional |
| Organism |
Medicago truncatula |
| Cultivar |
Jemalong A17 |
| Tissue type |
Late seeds |
| Developmental stage |
Immature seeds, collected from pods ranging in age from 25 to 35 days after pollination. |
| Vector |
pBluescript SK- |
| Lab. host |
E.coli XLOLR |
| Cloning site R1 (5') |
EcoRI |
| Cloning site R2 (3') |
XhoI |
| 5' Adaptor sequence |
5'-AATTCGGCACGAGG-3' |
| 3' Adaptor sequence |
5'-GAGAGAGAGAGAGAGAGAACTAGTCTCGAGT(17)-3' |
| Description |
Immature seeds, collected from pods ranging in age from 25 to 35 days after pollination, were harvested from greenhouse-grown plants. Seed were removed and separated from pod walls and immediately frozen in liquid nitrogen. Seeds throughout the age range were pooled for mRNA extraction. cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using ExAssist helper phage and propagated in XLOLR cells. |
| 5' Sequencing primer |
SKmod |
| 5' Sequencing primer sequence |
CTA gAA CTA gtg gAT CC |
| 3' Sequencing primer |
|
| 3' Sequencing primer sequence |
|
| Other sequencing primer |
|
| Other sequencing primer sequence |
|
| Potential source of contaminant |
|
| Additional comments |
|
| Library developer |
Michael A. Grusak |
| Publication reference |
(GenBank) ESTs from late stage developing seeds of Medicago truncatula.
Grusak,M.A., Samac,D., Town,C.D., Van Aken,S., Utterback,T., Cheung,F., Fraser,C.M. 2002. Unpublished |