| Title |
ESTs from Medicago truncatula roots infected with Meloidogyne incognita nematode |
| Library name |
BNIR |
| Library type |
EST, 5'-3' unidirectional |
| Organism |
Medicago truncatula |
| Cultivar |
Jemalong A17 |
| Tissue type |
Roots |
| Developmental stage |
3 week old roots 3 days after infection with Meloidogyne incognita second stage larvae. |
| Vector |
pBluescript SK- |
| Lab. host |
E.coli XLOLR |
| Cloning site R1 (5') |
EcoRI |
| Cloning site R2 (3') |
XhoI |
| 5' Adaptor sequence |
5'-AATTCGGCACGAGG-3' |
| 3' Adaptor sequence |
5'-GAGAGAGAGAGAGAGAGAACTAGTCTCGAGT(17)-3' |
| Description |
cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Unizap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using ExAssist helper phage and propagated in XLOLR cells. |
| 5' Sequencing primer |
SKmod |
| 5' Sequencing primer sequence |
CTA gAA CTA gtg gAT CC |
| 3' Sequencing primer |
|
| 3' Sequencing primer sequence |
|
| Other sequencing primer |
|
| Other sequencing primer sequence |
|
| Potential source of contaminant |
|
| Additional comments |
|
| Library developer |
DM Bird |
| Publication reference |
(GenBank) ESTs from roots of Medicago truncatula after infection with the nematode Meloidogyne incognita.
Bird,D., Koltai,H., Samac,D., Town,C.D., Van Aken,S., Utterback,T., Cheung,F., Tsai,J., Fraser,C.M.. 2002. Unpublished |