| Title |
ESTs from developing reproductive tissues (seeds) of Medicago truncatula |
| Library name |
GESD |
| Library type |
EST, 5'-3' unidirectional |
| Organism |
Medicago truncatula |
| Cultivar |
Jemalong A17 |
| Tissue type |
Immature seeds |
| Developmental stage |
Immature seeds, collected from pods ranging in age from 11 to 19 days after pollination, were harvested from greenhouse-grown plants. Seeds were removed and separated from pod walls and were immediately frozen in liquid nitrogen. Seeds throughout the age range were pooled for mRNA extraction. |
| Vector |
pBluescript SK- |
| Lab. host |
E.coli XLOLR |
| Cloning site R1 (5') |
EcoRI |
| Cloning site R2 (3') |
XhoI |
| 5' Adaptor sequence |
5'-AATTCGGCACGAGG-3' |
| 3' Adaptor sequence |
5'-GAGAGAGAGAGAGAGAGAACTAGTCTCGAGT(17)-3' |
| Description |
cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Uni-Zap XR vector from Stratagene and packaged using the Gigapack III Gold packaging extracts. Plasmids containing inserts were excised from the recombinant lambda-Zap phage using ExAssist helper phage and propagated in XLOLR cells. |
| 5' Sequencing primer |
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| 5' Sequencing primer sequence |
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| 3' Sequencing primer |
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| 3' Sequencing primer sequence |
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| Other sequencing primer |
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| Other sequencing primer sequence |
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| Potential source of contaminant |
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| Additional comments |
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| Library developer |
Michael A. Grusak, Deborah Samac |
| Publication reference |
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