| Title |
ESTs from roots of Medicago truncatula 24 hours after inoculation with Sinorhizobium meliloti |
| Library name |
KV1 |
| Library type |
EST, 5'-3' unidirectional |
| Organism |
Medicago truncatula |
| Tissue type |
Seedling roots |
| Developmental stage |
24 hours post-inoculation with Sinorhizobium meliloti |
| Vector |
pBluescript SK- |
| Lab. host |
E. coli XLOLR |
| Cloning site R1 (5') |
EcoRI |
| Cloningsite R2 (3') |
XhoI |
| 5' Adaptor sequence |
5'-AATTCGGCACGAGG-3' |
| 3' Adaptorsequence |
5'-GAGAGAGAGAGAGAGAGAACTAGTCTCGAGT(17)-3' |
| Description |
cDNA was prepared from polyA+ enriched RNA. The cDNA was directionally ligated into the Uni-Zap XR vector from Stratagene and packaged using the Gigapack III Gold packaging extracts. Plasmids containing inserts were excised from the recombinant lambda-Zap phage using ExAssist helper phage and propagated in XLOLR cells. |
| 5' Sequencing primer |
SKmod |
| 5' Sequencing primer sequence |
5'-CTA gAA CTA gtg gATC CC-3' |
| 3' Sequencing primer |
|
| 3' Sequencing primer sequence |
|
| Other sequencing primer |
|
| Other sequencing primer sequence |
|
| Potential source of contaminant |
|
| Additional comments |
|
| Library developer |
Kate VandenBosch |
| Publication reference |
|