| Title |
ESTs from roots of Medicago truncatula after inoculation with Phytophthora medicaginis |
| Library name |
DSIR |
| Library type |
EST, 5'-3' unidirectional |
| Organism |
Medicago truncatula |
| Tissue type |
Roots infected with Phytophthora medicaginis |
| Developmental stage |
Roots harvested at 10 days post inoculation with Phytophthora medicaginis |
| Vector |
pBluescript SK- |
| Lab. host |
E. coli XLOLR |
| Cloning site R1 (5') |
EcoRI |
| Cloning site R2 (3') |
XhoI |
| 5' Adaptor sequence |
5'-AATTCGGCACGAGG-3' |
| 3' Adaptor sequence |
5'-GAGAGAGAGAGAGAGAGAACTAGTCTCGAGT(17)-3' |
| Description |
cDNA was prepared from polyA+ enriched RNA from roots harvested at 10 days post inoculation with Phytophthora medicaginis. The cDNA was directionally ligated into the Uni-Zap XR vector from Stratagene and packaged using Gigapack III Gold packaging extracts. Plasmids containing cDNA inserts were excised from the recombinant lambda-Zap phage using Ex-Assist helper phage and propagated in SOLR cells. |
| 5' Sequencing primer |
SKmod |
| 5' Sequencing primer sequence |
5'-CTA gAA CTA gtg gATC CC-3' |
| 3' Sequencing primer |
|
| 3' Sequencing primer sequence |
|
| Other sequencing primer |
|
| Other sequencing primer sequence |
|
| Potential source of contaminant |
EST may be of fungal origin |
| Additional comments |
|
| Library developer |
Deborah Samac |
| Publication reference |
|